Gene expression analysis using the CFX Manager software. Quickly start, repeat, or begin analyzing a run in three clicks or fewer from the new Startup Wizard in the CFX Manager software.įig. Graph shows 96 replicates of 100 µl reactions. IL-1β plasmid template was diluted to 10 5 copies/reaction and amplified in the presence of a FAM-labeled detection probe with IQ™ supermix. Cq, quantification cycle RFU, relative fluorescence units.įig.
2012/10/color_rectangle_darkblue.jpg” style=”width: 7px height: 7px float:none display: inline margin-right: 0px ” /> FAM/cyclophilin /2012/10/color_rectangle_green.jpg” style=”width: 7px height: 7px float:none display: inline margin-right: 0px ” /> HEX/ GAPDH /2012/10/color_rectangle_red.jpg” style=”width: 7px height: 7px float:none display: inline margin-right: 0px ” /> Texas Red/actin /2012/10/color_rectangle_purple.jpg” style=”width: 7px height: 7px float:none display: inline margin-right: 0px ” /> Cy5/tubulin /2012/10/color_rectangle_darkred.jpg” style=”width: 7px height: 7px float:none display: inline margin-right: 0px ” /> Quasar 705/IL-1β F, standard curves generated from data in A–E, reaction efficiencies range from 100 to 102%.
A–E, fluorescence data from a series of tenfold dilutions of plasmid DNA (10 9–10 3 copies) amplified using reporter dyes to monitor five targets in a 75 µl reaction volume. Linearity of five-target multiplex detection. Runs can be quickly and easily set up using the intuitive LCD touch screen menus (Figure 3) and amplification traces can be viewed on the LCD screen in real time while a run is in progress (Figure 4) providing a truly stand-alone qPCR system.įig. The CFX96 Touch Deep Well system includes the easy-to-use C1000 Touch™ chassis to create a truly stand-alone qPCR system. The system also incorporates a built-in thermal gradient for easy assay optimization in a single run. This sample block heats and cools more quickly than standard blocks, so average ramp rates are increased and overall run times are reduced. In addition to using industry-leading optical technology, the system offers excellent thermal performance and uniformity for large-volume reactions by using Bio-Rad’s patented reduced-mass sample block (Figure 2). The optical system scans just above the sample plate to illuminate and detect from each well with high sensitivity and no cross talk. The system can accurately discriminate up to five targets in a single reaction well by using Bio-Rad’s solid-state optical technology (Figure 1). The new CFX96 Touch Deep Well real-time PCR detection system joins Bio-Rad’s real-time PCR detection system family as the system for large-volume qPCR reactions (up to 125 µl).
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